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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pLKO.1-Nrf2 shRNA
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pLKO.1-Nrf2 shRNA Plasmid?
Catalog No. PVTB00580-3a
Vector Backbone pLKO.1-Puro
Backbone manufacturer Addgene
Vector type Lentiviral interference
Backbone size w/o insert 7091bp
Cloning site 5 Age I
Site destroyed during cloning YES
Cloning site 3 EcoR I
Bacterial resistance Ampicillin
Growth strain Stbl3
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Puromycin
Background Mammalian shRNA Knockdown vector of Homo sapiens nuclear factor, erythroid 2 like 2
Gene Information
Alternative Gene Name NFE2L2; HEBP1
Insert Gene name Nrf2 shRNA?
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文献和实验,然后我收集这些上清直接去感染肝癌细胞,24小时候也 能看到至少80%的细胞表达GFP,说明包装过程中所用的包装质粒,试剂以及细胞应该没 有问题。我现在有几个疑问,请有经验的战友指点: 1)师姐留给我的protocol上,转染HEK293FT的方法如下,以10cmdish为例: prepare the following transfection mix: 10.0 ug PLKO.1 shRNA plasmid 10.5 ug pLP1 10.5 ug pLP2 9.0 ug pVSVG
Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
RNA interference is widely used for loss-of-function studies in mammalian cells. As an alternative to the transfection of small RNAs, plasmid vectors have been developed to express short hairpin RNAs (shRNAs). We engineered the pHYPER shRNA
Inducible shRNA Lentiviral Vectors
-iT™ Inducible H1 Lentiviral RNAi System uses a vector-based approach to allow efficient generation of H1/TO RNAi cassettes for regulated expression of shRNA molecules in mammalian cells. For constitutive expression of shRNA molecules in mammalian cells, use
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