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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pLVX-mCherry-C1-EGFP-LC3B(Human)
- 库存:
100
- 供应商:
LSMBIO
- 规格:
2ug
pLVX-mCherry-C1-EGFP-LC3B(Human) Plasmid
Catalog No. PVTB00191-2e
Vector Backbone pLVX-mCherry-C1
Backbone manufacturer Clontech
Vector type Mammalian Expression
Backbone size w/o insert 8778bp
Cloning site 5 EcoR I
Site destroyed during cloning NO
Cloning site 3 BamH I
Bacterial resistance Ampicillin
Growth strain Stbl3
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Puromycin
Background Lentiviral expression of Homo sapiens microtubule associated protein 1 light chain 3 beta, fused with the mCherry protein at the N-terminus and the EGFP protein at the C-terminus
Gene Information
Alternative Gene Name MAP1LC3B,ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a
Insert Gene name LC3B
Insert size 378bp
Accessions NM_022818
Tag mCherry(N-term);EGFP(N-term)
Species Homo sapiens (human)
Gene ID 81631
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文献和实验PCDNA3.1(-)-flag-mTOR(human) PCDNA3.1(-)-Flag PCDNA3.1-3HA PCDNA3.1(+)/myc-His PCDNA3.1-myc-his B pLKO.1-EGFP PYES2.0 ProkII
EGFP绿色荧光蛋白 pEGFP-N1 哺乳细胞质粒 Kan 真核表达载体,表达EGFP绿色荧光蛋白 pEGFP-N3 哺乳细胞质粒 Kan pEGFP-C1 哺乳细胞质粒 Kan 真核表达载体,表达EGFP绿色
substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage preferences (5). Sequences flanking EGFP have been converted to a Kozak consensus translation
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