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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pHT43
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pHT43
Search name
pHT43,Plasmid pHT43,pHT43 vector
Packing 2ug
Promoter: Lac
Replicator: ColE1 ori
Plasmid classification: Bacillus subtilis vectors
Plasmid size: 8057bp
Resistance: Ampicillin/ chloramphenicol
Mark: Chl
Clone strain: DH5 alpha
Culture conditions: 37 DEG C, aerobic LB
Host: Bacillus subtilis
Induction: IPTG induction
Expression: Bacterial
Use: Bacillus subtilis
Sequence
LOCUS Exported File 8057 bp ds-DNA circular SYN 07-2-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pHT43
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8057)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-2-7
FEATURES Location/Qualifiers
source 1..8057
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1238..1254
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(1297..2379)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 2763..2787
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 6134..6238
/gene="bla"
/note="AmpR promoter"
CDS 6239..7099
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7270..7858
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac cacattaaaa
961 aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc ttttctttct
1021 tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc gccagtcact
1081 atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
1141 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
1201 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
1261 gagctcaggc cttaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
1321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
1381 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
1441 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
1501 aaaatcctgt ttgatggtgg ttgacggcgg gatataacat gagctgtctt cggtatcgtc
1561 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
1621 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
1681 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
1741 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
1801 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
1861 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
1921 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
1981 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
2041 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
2101 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
2161 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
2221 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
2281 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
2341 ggcatactct gcgacatcgt ataacgttac tggtttcatc aaaatcgtct ccctccgttt
2401 gaatatttga ttgatcgtaa ccagatgaag cactctttcc actatcccta cagtgttatg
2461 gcttgaacaa tcacgaaaca ataattggta cgtacgatct ttcagccgac tcaaacatca
2521 aatcttacaa atgtagtctt tgaaagtatt acatatgtaa gatttaaatg caaccgtttt
2581 ttcggaagga aatgatgacc tcgtttccac cggaattagc ttggtaccag ctattgtaac
2641 ataatcggta cgggggtgaa aaagctaacg gaaaagggag cggaaaagaa tgatgtaagc
2701 gtgaaaaatt ttttatctta tcacttgaaa ttggaaggga gattctttat tataagaatt
2761 gtggaattgt gagcggataa caattcccaa ttaaaggagg aaggatcaat gattcaaaaa
2821 cgaaagcgga cagtttcgtt cagacttgtg cttatgtgca cgctgttatt tgtcagtttg
2881 ccgattacaa aaacatcagc cgtaggatcc tctagagtcg acgtccccgg ggcagcccgc
2941 ctaatgagcg ggcttttttc acgtcacgcg tccatggaga tctttgtctg caactgaaaa
3001 gtttatacct tacctggaac aaatggttga aacatacgag gctaatatcg gcttattagg
3061 aatagtccct gtactaataa aatcaggtgg atcagttgat cagtatattt tggacgaagc
3121 tcggaaagaa tttggagatg acttgcttaa ttccacaatt aaattaaggg aaagaataaa
3181 gcgatttgat gttcaaggaa tcacggaaga agatactcat gataaagaag ctctaaaact
3241 attcaataac cttacaatgg aattgatcga aagggtggaa ggttaatggt acgaaaatta
3301 ggggatctac ctagaaagcc acaaggcgat aggtcaagct taaagaaccc ttacatggat
3361 cttacagatt ctgaaagtaa agaaacaaca gaggttaaac aaacagaacc aaaaagaaaa
3421 aaagcattgt tgaaaacaat gaaagttgat gtttcaatcc ataataagat taaatcgctg
3481 cacgaaattc tggcagcatc cgaagggaat tcatattact tagaggatac tattgagaga
3541 gctattgata agatggttga gacattacct gagagccaaa aaacttttta tgaatatgaa
3601 ttaaaaaaaa gaaccaacaa aggctgagac agactccaaa cgagtctgtt tttttaaaaa
3661 aaatattagg agcattgaat atatattaga gaattaagaa agacatggga ataaaaatat
3721 tttaaatcca gtaaaaatat gataagatta tttcagaata tgaagaactc tgtttgtttt
3781 tgatgaaaaa acaaacaaaa aaaatccacc taacggaatc tcaatttaac taacagcggc
3841 caaactgaga agttaaattt gagaagggga aaaggcggat ttatacttgt atttaactat
3901 ctccatttta acattttatt aaaccccata caagtgaaaa tcctctttta cactgttcct
3961 ttaggtgatc gcggagggac attatgagtg aagtaaacct aaaaggaaat acagatgaat
4021 tagtgtatta tcgacagcaa accactggaa ataaaatcgc caggaagaga atcaaaaaag
4081 ggaaagaaga agtttattat gttgctgaaa cggaagagaa gatatggaca gaagagcaaa
4141 taaaaaactt ttctttagac aaatttggta cgcatatacc ttacatagaa ggtcattata
4201 caatcttaaa taattacttc tttgattttt ggggctattt tttaggtgct gaaggaattg
4261 cgctctatgc tcacctaact cgttatgcat acggcagcaa agacttttgc tttcctagtc
4321 tacaaacaat cgctaaaaaa atggacaaga ctcctgttac agttagaggc tacttgaaac
4381 tgcttgaaag gtacggtttt atttggaagg taaacgtccg taataaaacc aaggataaca
4441 cagaggaatc cccgattttt aagattagac gtaaggttcc tttgctttca gaagaacttt
4501 taaatggaaa ccctaatatt gaaattccag atgacgagga agcacatgta aagaaggctt
4561 taaaaaagga aaaagagggt cttccaaagg ttttgaaaaa agagcacgat gaatttgtta
4621 aaaaaatgat ggatgagtca gaaacaatta atattccaga ggccttacaa tatgacacaa
4681 tgtatgaaga tatactcagt aaaggagaaa ttcgaaaaga aatcaaaaaa caaataccta
4741 atcctacaac atcttttgag agtatatcaa tgacaactga agaggaaaaa gtcgacagta
4801 ctttaaaaag cgaaatgcaa aatcgtgtct ctaagccttc ttttgatacc tggtttaaaa
4861 acactaagat caaaattgaa aataaaaatt gtttattact tgtaccgagt gaatttgcat
4921 ttgaatggat taagaaaaga tatttagaaa caattaaaac agtccttgaa gaagctggat
4981 atgttttcga aaaaatcgaa ctaagaaaag tgcaataaac tgctgaagta tttcagcagt
5041 tttttttatt tagaaatagt gaaaaaaata taatcaggga ggtatcaata tttaatgagt
5101 actgatttaa atttatttag actggaatta ataattaaca cgtagactaa ttaaaattta
5161 atgagggata aagaggatac aaaaatatta atttcaatcc ctattaaatt ttaacaaggg
5221 ggggattaaa atttaattag aggtttatcc acaagaaaag accctaataa aatttttact
5281 agggttataa cactgattaa tttcttaatg ggggagggat taaaatttaa tgacaaagaa
5341 aacaatcttt taagaaaagc ttttaaaaga taataataaa aagagctttg cgattaagca
5401 aaactcttta ctttttcatt gacattatca aattcatcga tttcaaattg ttgttgtatc
5461 ataaagttaa ttctgttttg cacaaccttt tcaggaatat aaaacacatc tgaggcttgt
5521 tttataaact cagggtcgct aaagtcaatg taacgtagca tatgatatgg tatagcttcc
5581 acccaagtta gcctttctgc ttcttctgaa tgtttttcat atacttccat gggtatctct
5641 aaatgatttt cctcatgtag caaggtatga gcaaaaagtt tatggaattg atagttcctc
5701 tctttttctt caactttttt atctaaaaca aacactttaa catctgagtc aatgtaagca
5761 taagatgttt ttccagtcat aatttcaatc ccaaatcttt tagacagaaa ttctggacgt
5821 aaatcttttg gtgaaagaat ttttttatgt agcaatatat ccgatacagc accttctaaa
5881 agcgttggtg aatagggcat tttacctatc tcctctcatt ttgtggaata aaaatagtca
5941 tattcgtcca tctacctatc ctattatcga acagttgaac tttttaatca aggatcagtc
6001 ctttttttca ttattcttaa actgtgctct taactttaac aactcgattt gtttttccag
6061 atctcgaggg taactagcct cgccgatccc gcaagaggcc cggcagtcag gtggcacttt
6121 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
6181 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
6241 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
6301 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
6361 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
6421 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
6481 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
6541 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
6601 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
6661 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
6721 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
6781 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
6841 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6901 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6961 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
7021 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
7081 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
7141 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
7201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
7261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
7321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
7381 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
7441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
7501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
7561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
7621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
7921 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
7981 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
8041 gcgcccaata cgcatgc
//
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文献和实验CD43 分子 CD43 常用单克隆抗体或代号： OTH71C5,G19- 1;(Leukosialin,Sialophorin) 主要表达细胞： T,G,M [NL] 分子质量(kDa)和结构： gp95- 135 功 能： T细胞活化、增殖和粘附,与CD54结合 CD43 Aka leukosialin, sialophorin Appears
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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