重组人EPO-α北京价格

特别提示：包括重组人EPO-α在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组人EPO-α
英文名称：Recombinant Human Erythropoietin-alpha
产品货号：GS4435
产品规格：500IU

分子量：Mature human EPO,containing 166 amino acid residues,has a predicted molecular mass of approximately 21kDa.As a result of glycosylation,the recombinant protein migrates with an apparent molecular mass of 36-40 kDa in SDS-PAGE.
储存条件：冷冻（-20℃）

概述

Erythropoietin(EPO),a glycoprotein produced primarily by the kidney,is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.The production of EPO by kidney cells is increased in response to hypoxia or anemia.Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients.The cDNAs for EPO have been cloned from human,murine,canine,etc.The mature proteins from the various species are highly conserved,exhibiting greater than 80%sequence identity at the amino acid level.Human EPO cDNA encodes a 193 amino acid residue precursor protein that is processed to yield a 165 amino acid residue mature protein.EPO contains one O-linked and three N-linked glycosylation sites.Glycosylation of EPO is required for EPO biological activities in vivo.EPO exhibits structural as well as amino sequence identity to the amino terminal 153 amino acid region of thrombopoietin.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞98%by SDS-PAGE and HPLC analyses.
来源	CHO
生物活性	Fully biologically active when compared to standard.The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 1.5×105 IU/mg.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in sodium citrate buffer(1 liter of ddH2O containing 5.9gof sodium citrate,5.8 g of sodium chloride and 0.06 g of citric acid).
内毒素	Less than 0.01 EU/μg of rHuEPO-α as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

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