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- 详细信息
- 文献和实验
- 技术资料
- 库存:
109
- 英文名:
Recombinant Rat Interleukin-5
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
2μg
特别提示:包括重组大鼠IL-5在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组大鼠IL-5
英文名称:Recombinant Rat Interleukin-5
产品货号:GS4706
产品规格:2μg
分子量:Approximately 26.2kDa,a disulfide-linked homodimeric protein containing two 113 amino acids.
储存条件:冷冻(-20℃)
概述
IL-5,also named B-cell differentiation factor I,eosinophil differentiation factor and TRF,is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3,IL-4,and granulocyte-macrophage colony-stimulating factor(GM-CSF),which are often co-expressed in TH2 cells.Through binding to the IL-5 receptor,IL-5 stimulates B cell growth and increases immunroglobulin secretion.It is also a key mediator in eosinophil activation.Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma.Rat IL-5 is a 132-amino acid(115 in human,133 in the mouse)-long TH2 cytokine that is part of the hematopoietic family.Unlike other members of this cytokine family(namely IL-3 and GM-CSF),this glycoprotein in its active form is a homodimer.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >98%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5ng/ml,corresponding to a specific activity of>2.0×106 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rRtIL-5 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV |
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文献和实验图上查出相应 IL-5 含量。 试剂盒性能 1. 灵敏度 : 最小的 IL-5 检测浓度小于 16pg/ml 。 2. 特异性: 可同时检测重组或天然的大鼠 IL-5 。 不与 大鼠其它细胞因子有交叉反应。 3. 重复性:板内、板见变异系数均小于10 %。 注意事项 1. 以上标准孔及待测样品均建议做复孔,每次测定应同时做标准曲线。
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