- ￥980
- LSM Bio
- CHC00039
- 2025年07月14日
企业认证
相关产品推荐更多 >
LBA4404 感受态细胞(电转)/ LBA4404 Electro Agrobacterium Express Competent Cells
￥1180Ar A4 感受态细胞(化转)/ Ar A4 chemically Agrobacterium Express Competent Cells
￥980Tuner (DE3) 感受态细胞(化转)/ Tuner (DE3) chemically E.coli Express Competent Cells
￥980BJ5183-AD-1 感受态细胞(化转)/ BJ5183-AD-1 chemically Competent Cells
￥980JM109 (DE3) 感受态细胞(化转)/ JM109 (DE3) chemically E.coli Express Competent Cells
￥980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
DH10Bac chemically E.coli Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
DH10BAC Chemically Competent Cells
Packing 10 x 0.1 ml/tube
Store at –80°C
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara,leu)7697 galU galK λ- rpsL nupG /pMON14272 / pMON7124
Alternative name
DH10BAC chemically Competent Cells,DH10BAC Competent E.coli,DH10BAC Competent Cells, DH10BAC chemically E.coli Express Competent Cells
Transformation Protocol
1.Please store DH10BAC Chemically Competent Cell in -80°C for late use.
2. Thaw a tube of DH10BAC Chemically Competent Cell锛?00μl 锛塷n ice.
3. Add 1µl plasmid DNA or 10µl ligation product to the cell mixture. Carefully flick the tube 4–5 times to mix cells and DNA. Do not vortex.
4.Place the mixture on ice for 30 minutes. Do not mix.
5. Heat shock at exactly 42°C for exactly 90 seconds and then immediately transfer the tube back into ice for 5min. Do not mix.
6. Place on ice for 5 minutes. Do not mix.
7. Add 800ul LB then put on the Constant temperature shaker (150 rpm) for 45min,Warm selection plates to 37°C.
8. Pellet the mixture by centrifugation at 4000rpm/min for 3min. Pour off about 200ul of cell supernatant, then resuspend the cell pellet in the remaining medium by gently vortexing the tube.
9.Spread the cell on the agar plates by antibiotic, then place it on 37°C for 1hour.
10. Incubate overnight at 37°C.
Caution:
1.STORAGE AND HANDLING: Competent cells should be stored at
–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.
2. This product is FOR RESEARCH USE ONLY!
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
); 1000 X stock = Spermidine 750 mM (1.9g/10ml) + Spermine 300 mM (1.04g/10 ml)] PREPARATION OF COMPETENT CELLS AND BAC ELECTROPORATION 1. PREPARATION OF CELLS 1) Inoculate flasks of SOB (without Mg++) by diluting a fresh saturated
a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
技术资料暂无技术资料 索取技术资料
