- ¥7619
- Gibco
- A1113802
- 美国
- 2025年08月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-5 to -20°C
- 规格：
A1113802
描述
Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.Puromycin is widely used in cell biology as a selection antibiotic in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 7.2–7.5).
Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.
See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection
See recommendations for working concentrations for selection antibiotics.
Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
For Research Use Only. Not for use in diagnostic procedures.
规格
| Validated Application: | Eukaryotic Selection⁄Stable Cell Line Generation |
|---|---|
| Agent: | Puromycin |
| Form: | Liquid |
| Reagent Type: | Antibiotic (Selective) |
| Concentration: | 10 mg⁄ml |
| Formula Weight: | 544.43 g⁄mole |
| Product Line: | Gibco® |
| Product Size: | 20 mL |
| Quantity: | 1 bottle |
| Sterility: | Sterile |
| Shipping Condition: | Dry Ice |
内容及储存
Storage conditions: -5 to -20°CShipping conditions: Frozen
Shelf life: 12 months from date of manufacture
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文献和实验Determining Relative Transduction of Adherent Cells
实验原理 MISSION® TurboGFP™ control particles enable fast determination of the appropriate transduction conditions for efficient transduction of cells with lentiviral particles. Note: The CMV promoter is known to function
Lentiviral Transduction Protocol for Human Embryonic Stem Cells
设备 1. 24-well culture dishes (Product No. CLS3526) 2. Matrigel™ basement membrane 实验材料 MISSION® shRNA lentiviral particles 实验步骤 Prior to transduction — establish the Puromycin Kill Curve
website for further details and for obtaining and MTA. Use passage number Trypsin/EDTA (Gibco) Retroviral plasmid DNA (usually based on MMLV based with LTRs, the packaging signal sequence and containing the gene of interest, a promotor
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