RPE65| Human Retinoid isomeroh

Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina

- retinyl ester (all-trans- RE) or an all-trans- retinol (all-trans- ROL). In RPE cells, this step is carried out by an enzyme called Rpe65 isomerase. This chapter provides methods for assaying Rpe65 isomerase. Although Rpe65 utilizes

Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle

and retinoid content of the retina can provide important insights into the mechanisms of disease initiation and progression. Here, we use the protein RPE65 as an example to describe generally applicable methods for determining

Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy

activation by light. Many different mutations in genes encoding retinoid cycle proteins can cause a variety of human blinding diseases. Two-photon microscopy is an evolving, non-invasive, and repetitive imaging technology