Human LMAN1 ELISA Kit
Product Name:Human LMAN1 ELISA Kit
Packing:96T
Catalog No.:ELA-E9737h
Gene Name:LMAN1
Detect Range:78-5000 pg/mL
Sensitivity:31pg/mL
Target Protein Name:Human Protein ERGIC-53
Alternative Name:Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, LMAN1, ERGIC53, F5F8D
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human LMAN1 ELISA Kit allows for the in vitro quantitative determination of Human Protein ERGIC-53 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human LMAN1 ELISA Kit has been pre-coated with an LMAN1 antibody specific to Human Protein ERGIC-53 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein ERGIC-53 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein ERGIC-53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Protein ERGIC-53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!