Human BCAM ELISA Kit
Product Name:Human BCAM ELISA Kit
Packing:96T
Catalog No.:ELA-E9357h
Gene Name:BCAM
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Human Basal cell adhesion molecule
Alternative Name:Basal cell adhesion molecule, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, BCAM, LU, MSK19, CD239
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human BCAM ELISA Kit allows for the in vitro quantitative determination of Human Basal cell adhesion molecule concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human BCAM ELISA Kit has been pre-coated with an BCAM antibody specific to Human Basal cell adhesion molecule .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Basal cell adhesion molecule and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Basal cell adhesion molecule, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Basal cell adhesion molecule in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!