Target Protein Name:Human Tyrosine-protein kinase Tec
Alternative Name:Tyrosine-protein kinase Tec, TEC, PSCTK4 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human TEC ELISA Kit allows for the in vitro quantitative determination of Human Tyrosine-protein kinase Tec concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human TEC ELISA Kit has been pre-coated with an TEC antibody specific to Human Tyrosine-protein kinase Tec .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Tyrosine-protein kinase Tec and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Tyrosine-protein kinase Tec, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Tyrosine-protein kinase Tec in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!