- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- EF008280
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human C3 /C3b /C3c
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
C3 /C3b /C3c ELISA KIT|Human
Catalog EF008280
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
C3 /C3b /C3c ELISA KIT Sensitivity< 0.094ng/ml
Application C3 /C3b /C3c ELISA KIT|Human is for detecting the concentration of Human C3 /C3b /C3c in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of C3 /C3b /C3c ELISA KIT|Human
The microtiter plate provided in C3 /C3b /C3c ELISA KIT|Human has been pre-coated with an Human C3 /C3b /C3c antibody specific to Human C3 /C3b /C3c. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human C3 /C3b /C3cantibody preparation specific for Human C3 /C3b /C3c then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human C3 /C3b /C3c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human C3 /C3b /C3cin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that C3 /C3b /C3c ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验C3是补体二条激活途径的关键分子，C3活化后裂解为C3a、C3b两个片段，C3a很快被血清羧肽酶N裂解为C3adesarg，C3b被H、I因子灭活为C3bi，经蛋白酶水解为C3c、C3d、C3dg等，检测这些裂解产物即可反映C3的活化程度。C3a的定量检测采用竞争性抑制放射免疫法。其原理为用“I标记C3（I-C3）”与待测样本、兔抗人C3a多克隆抗体一起孵育，待测样本中的C3a（未标记抗原）能竞争性地与抗C3a结合，使C3a复合物（B）增多，而“I-C3”游离出来（F），根据B%来判断待测样本
补体成分 C3(C3a/C3b) 补体成分：C3 血清浓度(μg/ml)：550-1200 分子量(kDa)：195 亚单位(链)及分子量(kDa)：α∶110；β∶85 激活产物：C3a；C3b 生物学活性：C3a 过敏毒素、趋化作用等。 C3b 组成CP、AP中的C3、C5转化
C3d的定量检测采用ELISA双抗体夹心法，其原理为抗C3d血清能与C3、C3b、C3bi发生交叉反应，根据C3SP与C3在不同浓度PEG中溶解度的不同，用11%PEG溶解待测样本中C3d，然后加至包被抗C3d反应板中，再依次加入HRP标记的抗C3d抗体和底物OPD/H202，用4mol/L H2S04终止反应，于492nm读取吸光度，C3d含量与其吸光度呈正相关。 1、10mmol/LEDTA抗凝正常人或病人全血，2500rpm离心10min,取血浆置-70℃保存。 2、C3d标准
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