- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- EF007439
- 2026年01月28日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human NK cell
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
NK cell ELISA KIT|Human
Catalog EF007439
Packing 48T/96T
Reactivity Human
Detect Range 0.625-40ng/ml
NK cell ELISA KIT Sensitivity< 0.375ng/ml
Application NK cell ELISA KIT|Human is for detecting the concentration of Human NK cell in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of NK cell ELISA KIT|Human
The microtiter plate provided in NK cell ELISA KIT|Human has been pre-coated with an Human NK cell antibody specific to Human NK cell. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human NK cellantibody preparation specific for Human NK cell then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NK cell, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human NK cellin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that NK cell ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus
Natural killer (NK) cell lines are difficult to transfect using standard techniques, which limits the ability to establish long-term knockdown of proteins with short-hairpin (sh)RNAs. We have developed a method to stably knockdown protein
the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
Lentiviral Gene Transduction in Human and Mouse NK Cell Lines
cells. We have standardized methods of lentiviral infection in human and mouse NK cell lines. We obtain transduction efficiencies of 15% in the NK-92 cell line and 30–40% in LNK, YT, and DERL7 cell lines. This method allows efficient and stable
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