- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产(香港)
- EF007437
- 2026年04月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human RF-IgM
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
RF-IgM ELISA KIT|Human
Catalog EF007437
Packing 48T/96T
Reactivity Human
Detect Range 4.69-300IU/ml
RF-IgM ELISA KIT Sensitivity< 2.813IU/ml
Application RF-IgM ELISA KIT|Human is for detecting the concentration of Human RF-IgM in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of RF-IgM ELISA KIT|Human
The microtiter plate provided in RF-IgM ELISA KIT|Human has been pre-coated with an Human RF-IgM antibody specific to Human RF-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human RF-IgMantibody preparation specific for Human RF-IgM then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human RF-IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human RF-IgMin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that RF-IgM ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验效应。假使在被测分子的不同位点上含有多个相同的决定簇，例如HBsAg的a决定簇，也可用针对此决定簇的同一单抗分别包被固相和制备酶结合物。但在HBsAg的检测中应注意亚型问题，HBsAg有adr、adw、ayr、ayw4个亚型，虽然每种亚型均有相同的a决定簇的反应性，这也是用单抗作夹心法应注意的问题。双抗体夹心法测抗原的另一注意点是类风湿因子（RF）的干扰。RF是一种自身抗体，多为IgM型，能和多种动物IgG的Fc段结合。用作双抗体夹心法检测的血清标本中如含有RF，它可充当抗原成份，同时与固相抗体和酶标
Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
人莱姆病IgM(Lyme-IgM)酶联免疫分析（ELISA）
人莱姆病 IgM(Lyme-IgM) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于检测人血清，血浆及相关液体样本中莱姆病IgM(Lyme-IgM) 水平。 实验原理： 本试剂盒采用双抗原夹心酶联免疫法（ ELISA ）测定标本中人莱姆病 IgM(Lyme-IgM) 。用纯化的抗原包被微孔板，制成固相抗原，可与样品中莱姆病 IgM(Lyme-IgM) 相结合 ，经洗
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