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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Ang III
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Ang III ELISA KIT|Human
Catalog EF006595
Packing 48T/96T
Reactivity Human
Detect Range 78.125-5000pg/ml
Ang III ELISA KIT Sensitivity< 46.875pg/ml
Application Ang III ELISA KIT|Human is for detecting the concentration of Human Ang III in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Ang III ELISA KIT|Human
The microtiter plate provided in Ang III ELISA KIT|Human has been pre-coated with an Human Ang III antibody specific to Human Ang III . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Ang III antibody preparation specific for Human Ang III then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Ang III , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Ang III in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Ang III ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Inducing RNAi with siRNA Cocktails Generated by RNase III
a number of long dsRNA substrates. Human GAPDH, La, and c-FOS dsRNA (200 bp) was prepared by in vitro transcription ( Silencer ™ siRNA Cocktail Kit (RNase III); See Materials and Methods). The dsRNA was digested using 1 U RNase III
Activation and Expansion of Human Treg Cells
from the magnet. (5)Resuspend the washed Dynabeads in the same volume of Buffer 1 as the initial volume of Dynabeads. 2. Isolation of Human Treg Cells For isolation of human Treg cells it is recommended to use the Dynal CD4 CD25 Treg Kit. 3. Expansion
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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