Application BCL2L1/BCL-X ELISA KIT|Human is for detecting the concentration of Human BCL2L1/BCL-X in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of BCL2L1/BCL-X ELISA KIT|Human
The microtiter plate provided in BCL2L1/BCL-X ELISA KIT|Human has been pre-coated with an Human BCL2L1/BCL-X antibody specific to Human BCL2L1/BCL-X . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human BCL2L1/BCL-X antibody preparation specific for Human BCL2L1/BCL-X then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human BCL2L1/BCL-X , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human BCL2L1/BCL-X in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that BCL2L1/BCL-X ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!