Application PTCH2 ELISA KIT|Human is for detecting the concentration of Human PTCH2 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of PTCH2 ELISA KIT|Human
The microtiter plate provided in PTCH2 ELISA KIT|Human has been pre-coated with an Human PTCH2 antibody specific to Human PTCH2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human PTCH2antibody preparation specific for Human PTCH2 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PTCH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human PTCH2in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that PTCH2 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!