- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 国产
- EF001782
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Hunan PIGS
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
PIGS ELISA KIT|Human
Catalog EF001782
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
PIGS ELISA KIT Sensitivity< 0.188ng/ml
Application PIGS ELISA KIT|Human is for detecting the concentration of Human PIGS in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of PIGS ELISA KIT|Human
The microtiter plate provided in PIGS ELISA KIT|Human has been pre-coated with an Human PIGS antibody specific to Human PIGS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human PIGSantibody preparation specific for Human PIGS then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PIGS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human PIGSin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that PIGS ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
部分。它们的错误折叠，如天然结构的丢失，是任何分析的一个重大问题，因为它会引起抗体结合部位、抗体的抗原结合区或抗原的表位的改变，这两者都是待测物结合所必需的。因此，捕获分子无法结合待测物，甚至可能由于暴露天然未暴露的氨基酸序列和结构而发生非特异性结合，导致诊断试验显示错误的结果。由于表面效应引起的空间结构和构象的改变，抗体在包被过程中会发生错误折叠。在ELISA微孔板中，只有3-10%的包被抗体具有良好的定向性和与待测物结合的功能活性。抗原包被也有类似的情况。在大多数情况下，很少量的活性成分就足以完成一次检测
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