- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 国产
- EF001748
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Hunan phospho-RCC1 Ser12
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
phospho-RCC1 Ser12 ELISA KIT|Human
Catalog EF001748
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
phospho-RCC1 Ser12 ELISA KIT Sensitivity< 0.188ng/ml
Application phospho-RCC1 Ser12 ELISA KIT|Human is for detecting the concentration of Human phospho-RCC1 Ser12 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of phospho-RCC1 Ser12 ELISA KIT|Human
The microtiter plate provided in phospho-RCC1 Ser12 ELISA KIT|Human has been pre-coated with an Human phospho-RCC1 Ser12 antibody specific to Human phospho-RCC1 Ser12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human phospho-RCC1 Ser12antibody preparation specific for Human phospho-RCC1 Ser12 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human phospho-RCC1 Ser12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human phospho-RCC1 Ser12in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that phospho-RCC1 Ser12 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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