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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Hunan Carbamylated Albumin
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Carbamylated Albumin ELISA KIT|Human
Catalog EF000639
Packing 48T/96T
Reactivity Human
Detect Range 12.5-800pg/ml
Carbamylated Albumin ELISA KIT Sensitivity< 7.5pg/ml
Application Carbamylated Albumin ELISA KIT|Human is for detecting the concentration of Human Carbamylated Albumin in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Carbamylated Albumin ELISA KIT|Human
The microtiter plate provided in Carbamylated Albumin ELISA KIT|Human has been pre-coated with an Human Carbamylated Albumin antibody specific to Human Carbamylated Albumin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Carbamylated Albuminantibody preparation specific for Human Carbamylated Albumin then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Carbamylated Albumin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Carbamylated Albuminin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Carbamylated Albumin ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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of the sample can be determined using a standard protein assay kit. Depending on the assay system, the lysis buffer used to prepare the bacterial proteins may give a background equivalent to approximately 5mg/ml of protein as determined from a standard curve
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