- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-E1213h
- 2026年01月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Calcitonin gene-related peptide 1,CGRP
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human Calcitonin gene- related peptide 1, CGRP ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Calcitonin gene-related peptide 1,Alpha-type CGRP,Calcitonin gene-related peptide I(CGRP-I), CALC1; CGRP; CGRP-I; CGRP1;
alpha-type CGRP; calcitonin 1; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; katacalcin;
calcitonin-related polypeptide alpha
Search name
Human Calcitonin gene-related peptide 1 ELISA Kit, Human CGRP ELISA Kit, Human Alpha-type CGRP ELISA Kit, Human
Calcitonin gene-related peptide I ELISA Kit, Human CGRP-I ELISA Kit, Human CALC1ELISA Kit, Human CGRP-I ELISA Kit,
Human CGRP1 ELISA Kit, Human calcitonin 1 ELISA Kit, Human calcitonin/calcitonin-related polypeptide alpha ELISA Kit,
Human katacalcin ELISA Kit, Human calcitonin-related polypeptide alpha ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human calcitonin gene-related peptide 1,CGRP concentrations
in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CGRP. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CGRP and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain CGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CGRP in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Characterization of CGRP Receptor Binding
for calcitonin gene‐related peptide (CGRP) and islet amyloid polypeptide (IAPP) in mammalian lung: Species variation and binding of truncated CGRP and IAPP. Endocrinology. 133:2351‐2361.
Production of Antisera Using Peptide Conjugates
of different crosslinking agents utilized for the coupling of peptides to carrier proteins; examples of each type are covered in this chapter. In the case of larger neuropeptides, such as calcitonin gene-related peptide (CGRP
in four different osteoblastic cell lines and in isolated neonatal mouse calvarial bone cells has been examined. In the rat osteosarcoma cell line UMR-106-01, vasoactive intestinal polypeptide (VIP, 0.001-1 microM), calcitonin gene-related peptide (CGRP, 0.3-30 nM
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