- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- EF000038
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human TA
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
TA ELISA KIT|Human
Catalog EF000038
Packing 48T/96T
Reactivity Human
Detect Range 4.688-300ng/ml
TA ELISA KIT Sensitivity< 2.813ng/ml
Application TA ELISA KIT|Human is for detecting the concentration of Human TA in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of TA ELISA KIT|Human
The microtiter plate provided in TA ELISA KIT|Human has been pre-coated with an Human TA antibody specific to Human TA . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human TA antibody preparation specific for Human TA then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human TA , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human TA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that TA ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
This protocol uses Promega''s pGEM-T kit (#A3600). PCR For TA cloning, it is optimal if the PCR primers have G''s at the 5'' end as this will maximize the probability of Taq polymerase adding the terminal A overhang
Solution 0.33 µl (in kit in -20℃ freezer) TOPO Vector 0.33 µl (in kit in -20℃ freezer) FINAL VOLUME2.00 µl 4. Incubate: Gently mix (do not use a pipettor!) the reactions and incubate at room temperature for 5 minutes. For large PCR
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