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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Rho GTPase-activating protein 15,ARHGAP15
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT
Product Name:Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT
Packing:96T
Catalog No.:ELI-52594h
Gene Name:ARHGAP15
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Rho GTPase-activating protein 15(ARHGAP15
Alternative Name:ARHGAP15,Rho GTPase-activating protein 15(ARHGAP15
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 15(ARHGAP15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 15(ARHGAP15 antibody specific to Rho GTPase-activating protein 15(ARHGAP15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 15(ARHGAP15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 15(ARHGAP15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 15(ARHGAP15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
., and Danuser, G. 2009. Coordination of Rho GTPase activities during cell protrusion. Nature 461:99‐103. Michaelson, D., Silletti, J., Murphy, G., D'Eustachio, P., Rush, M
Substrate Tablete(antibody developed in goat) St. Louis,Mo 63178, USAAffinity isolated antibody adsorbed Lot # 043H6178With bovine horse and human serum protein Cat # 0h176Lot # 055H4835Lot # A-2179Tween 80Laboratories ,Detroit,Michigan ,USA1 drop of Tween
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
., ). View Image Figure 19.15.2 Scheme for genetic selection for cyclic peptide inhibitors of protein‐protein interactions (Horswill et al
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