Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT
Product Name:Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT
Packing:96T
Catalog No.:ELI-52594h
Gene Name:ARHGAP15
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Rho GTPase-activating protein 15(ARHGAP15
Alternative Name:ARHGAP15,Rho GTPase-activating protein 15(ARHGAP15
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 15(ARHGAP15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 15, ARHGAP15 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 15(ARHGAP15 antibody specific to Rho GTPase-activating protein 15(ARHGAP15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 15(ARHGAP15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 15(ARHGAP15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 15(ARHGAP15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!