Product Name:Human Cornifin- A, SPRR1A ELISA KIT Packing:96T
Catalog No.:ELI-52890h
Gene Name:SPRR1A
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cornifin-A(SPRR1A
Alternative Name:SPRR1A,Cornifin-A(SPRR1A Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Cornifin- A, SPRR1A ELISA KIT allows for the in vitro quantitative determination of Cornifin-A(SPRR1A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Cornifin- A, SPRR1A ELISA KIT has been pre-coated with an Cornifin-A(SPRR1A antibody specific to Cornifin-A(SPRR1A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cornifin-A(SPRR1A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cornifin-A(SPRR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cornifin-A(SPRR1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!