- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-52472h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Radial spoke head 10 homolog B,RSPH10B
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human Radial spoke head 10 homolog B, RSPH10B ELISA KIT
Product Name:Human Radial spoke head 10 homolog B, RSPH10B ELISA KIT
Packing:96T
Catalog No.:ELI-52472h
Gene Name:RSPH10B
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Radial spoke head 10 homolog B(RSPH10B
Alternative Name:RSPH10B,Radial spoke head 10 homolog B(RSPH10B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Radial spoke head 10 homolog B, RSPH10B ELISA KIT allows for the in vitro quantitative determination of Radial spoke head 10 homolog B(RSPH10B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Radial spoke head 10 homolog B, RSPH10B ELISA KIT has been pre-coated with an Radial spoke head 10 homolog B(RSPH10B antibody specific to Radial spoke head 10 homolog B(RSPH10B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Radial spoke head 10 homolog B(RSPH10B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Radial spoke head 10 homolog B(RSPH10B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Radial spoke head 10 homolog B(RSPH10B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
works, is assessed by digesting, after step 6, with DNAse buffer for 10 min at room temperature and wash extensively with deionized water before step 7. B.3.3 Time considerations The protocol require 3-4 hours excluding
Dual- and Triple-Color Fluorospot
of plates can be done using a multichannel device. In washing steps not requiring sterile conditions (from “Detection of spots”, step 6 and forward), a regular ELISA plate washer with a washing head adjusted to ELISPOT/fluorospot plates can be used.
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