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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human UDP-glucuronosyltransferase 2B7,UGT2B7
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Human UDP- glucuronosyltransferase 2B7, UGT2B7 ELISA KIT
Product Name:Human UDP- glucuronosyltransferase 2B7, UGT2B7 ELISA KIT
Packing:96T
Catalog No.:ELI-51368h
Gene Name:UGT2B7
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:UDP-glucuronosyltransferase 2B7(UGT2B7
Alternative Name:UGT2B7,UDP-glucuronosyltransferase 2B7(UGT2B7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human UDP- glucuronosyltransferase 2B7, UGT2B7 ELISA KIT allows for the in vitro quantitative determination of UDP-glucuronosyltransferase 2B7(UGT2B7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human UDP- glucuronosyltransferase 2B7, UGT2B7 ELISA KIT has been pre-coated with an UDP-glucuronosyltransferase 2B7(UGT2B7 antibody specific to UDP-glucuronosyltransferase 2B7(UGT2B7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for UDP-glucuronosyltransferase 2B7(UGT2B7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UDP-glucuronosyltransferase 2B7(UGT2B7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of UDP-glucuronosyltransferase 2B7(UGT2B7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Glucuronidation of Fatty Acids and Prostaglandins by Human UDP-Glucuronosyltransferases
glucuronidation from both human liver micro-somes and human recombinant UDP-glucuronosyltransferase 2B7 (UGT2B7) are presented. Several unique methods for synthesis of FAs that are not commercially available are also described. Moreover, comprehensive methods
Phenotyping UDP-Glucuronosyltransferases (UGTs) Involved in Human Drug Metabolism: An Update
Glucuronidation, catalyzed by the UDP-glucuronosyltransferases (UGT), is a major drug clearance mechanism in humans and other mammalian species. UGT reaction phenotyping involves determining which of the 19 known human UGTs are primarily
In Vitro Identification of UDP-Glucuronosyltransferases (UGTs) Involved in Drug Metabolism
Glucuronidation catalyzed by the UDP-glucuronosyltransferases (UGTs) is a major pathway for drug metabolism and elimination in humans. Identification of the UGTs responsible for glucuronidation of existing and novel drugs will assist
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