- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-51360h
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ubiquitin carboxyl-terminal hydrolase 31,USP31
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human Ubiquitin carboxyl- terminal hydrolase 31, USP31 ELISA KIT
Product Name:Human Ubiquitin carboxyl- terminal hydrolase 31, USP31 ELISA KIT
Packing:96T
Catalog No.:ELI-51360h
Gene Name:USP31
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Ubiquitin carboxyl-terminal hydrolase 31(USP31
Alternative Name:USP31,Ubiquitin carboxyl-terminal hydrolase 31(USP31
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin carboxyl- terminal hydrolase 31, USP31 ELISA KIT allows for the in vitro quantitative determination of Ubiquitin carboxyl-terminal hydrolase 31(USP31 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin carboxyl- terminal hydrolase 31, USP31 ELISA KIT has been pre-coated with an Ubiquitin carboxyl-terminal hydrolase 31(USP31 antibody specific to Ubiquitin carboxyl-terminal hydrolase 31(USP31 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase 31(USP31 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase 31(USP31, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin carboxyl-terminal hydrolase 31(USP31 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
) 6% to 8% SDS‐PAGE minigels (unit 6.1 ) Anti‐ubiquitin antibody ( protocol 31 ; for immunoblot detection) Boiling water bath Additional reagents and equipment for SDS
【分享】Human press METHODS IN MOLECULAR BIOLOGY
.Ubiquitin-Proteasome Protocols Edited by Patterson, Cam 2005 302.Handbook of ELISPOT Methods and Protocols Edited by Kalyuzhny, Alexander E. 2005 303.NanoBiotechnology Protocols Edited by Rosenthal, Sandra J 2005 304.Human Retrovirus Protocols Virology
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
High‐molecular‐weight human genomic DNA, prepared from peripheral blood using commercial kit (e.g., Puregene; Gentra Systems) 10 U/µl restriction endonucleases (Taq
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