Target Protein Name:Splicing factor U2AF 35 kDa subunit(U2AF1
Alternative Name:U2AF1,Splicing factor U2AF 35 kDa subunit(U2AF1 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Splicing factor U2AF 35 kDa subunit, U2AF1 ELISA KIT allows for the in vitro quantitative determination of Splicing factor U2AF 35 kDa subunit(U2AF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Splicing factor U2AF 35 kDa subunit, U2AF1 ELISA KIT has been pre-coated with an Splicing factor U2AF 35 kDa subunit(U2AF1 antibody specific to Splicing factor U2AF 35 kDa subunit(U2AF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Splicing factor U2AF 35 kDa subunit(U2AF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Splicing factor U2AF 35 kDa subunit(U2AF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Splicing factor U2AF 35 kDa subunit(U2AF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!