- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- ELI-50461h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Gamma-crystallin N,CRYGN
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Human Gamma- crystallin N, CRYGN ELISA KIT
Product Name:Human Gamma- crystallin N, CRYGN ELISA KIT
Packing:96T
Catalog No.:ELI-50461h
Gene Name:CRYGN
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Gamma-crystallin N(CRYGN
Alternative Name:CRYGN,Gamma-crystallin N(CRYGN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Gamma- crystallin N, CRYGN ELISA KIT allows for the in vitro quantitative determination of Gamma-crystallin N(CRYGN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Gamma- crystallin N, CRYGN ELISA KIT has been pre-coated with an Gamma-crystallin N(CRYGN antibody specific to Gamma-crystallin N(CRYGN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gamma-crystallin N(CRYGN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gamma-crystallin N(CRYGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Gamma-crystallin N(CRYGN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig 分五类，即 IgG、IgA、IgM、IgD 和 IgE。与免疫测定有关的 Ig 主要为 IgG 和 IgM。Ig 由两个轻链（L）和两个重链（H）的单体组成。Ig 的轻链是相同的，有 κ（kappa）和 λ（Lambda）两种型别。五类 Ig 的重链结构不同，这决定了它们的抗原性也不同。IgG 和 IgM 的重链分别称为 γ（gamma）链和 μ（mu ）链。 重链和轻链的 N 端的氨基酸排列顺序
人 Neuritin 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本 中Neuritin 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人 Neuritin 水平。用纯化的 Neuritin 抗体 包被微孔板，制成固相抗体，往包被单抗的微孔中加入 Neuritin ，再与 HRP 标记的 Neuritin 抗体结合，形成抗体
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
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