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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Signal transduction protein CBL-C,CBLC
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Human Signal transduction protein CBL- C, CBLC ELISA KIT
Product Name:Human Signal transduction protein CBL- C, CBLC ELISA KIT
Packing:96T
Catalog No.:ELI-50273h
Gene Name:CBLC
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Signal transduction protein CBL-C(CBLC
Alternative Name:CBLC,Signal transduction protein CBL-C(CBLC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Signal transduction protein CBL- C, CBLC ELISA KIT allows for the in vitro quantitative determination of Signal transduction protein CBL-C(CBLC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Signal transduction protein CBL- C, CBLC ELISA KIT has been pre-coated with an Signal transduction protein CBL-C(CBLC antibody specific to Signal transduction protein CBL-C(CBLC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Signal transduction protein CBL-C(CBLC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Signal transduction protein CBL-C(CBLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Signal transduction protein CBL-C(CBLC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature 312:315‐321. Berridge, M.J., Dawson, R.M.C., Downes, C.P., Heslop, J.P., and Irvine, R.F
of itself and other adaptor proteins that mediate the activation of its downstream signal molecules such as mitogen-activated protein (MAP) kinase and phosphatidylinositol 3-kinase (PI3K ) (8 ,9) . These cascades can then regulate nuclear and cytoplasmic events controlling
Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
at the molecular and sub-cellular level. This complexity in cell signal pattern begins with ligand binding to the receptor and its coupling to a variety of different effector systems. These signal transduction cascades within a cell involve a very complex series
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