Human G2/mitotic- specific cyclin- B1, CCNB1 ELISA KIT
Product Name:Human G2/mitotic- specific cyclin- B1, CCNB1 ELISA KIT Packing:96T
Catalog No.:ELI-50240h
Gene Name:CCNB1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:G2(mitotic-specific cyclin-B1(CCNB1
Alternative Name:CCNB1,G2(mitotic-specific cyclin-B1(CCNB1 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human G2/mitotic- specific cyclin- B1, CCNB1 ELISA KIT allows for the in vitro quantitative determination of G2(mitotic-specific cyclin-B1(CCNB1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human G2/mitotic- specific cyclin- B1, CCNB1 ELISA KIT has been pre-coated with an G2(mitotic-specific cyclin-B1(CCNB1 antibody specific to G2(mitotic-specific cyclin-B1(CCNB1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for G2(mitotic-specific cyclin-B1(CCNB1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G2(mitotic-specific cyclin-B1(CCNB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of G2(mitotic-specific cyclin-B1(CCNB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!