Human Cytochrome P450 11B1,mit

Human Cytochrome P450 11B1 mitochondrial, CYP11B1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cyp11b1,CPN1; CYP11B; FHI; P450C11; CYPXIB1; cytochrome P-450c11; cytochrome P450 11B1, mitochondrial; cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 1; cytochrome P450C11; cytochrome p450 XIB1; steroid 11-beta-hydroxylase; steroid 11-beta-monooxygenase; cytochrome P450, family 11, subfamily B, polypeptide 1

Search name

Human Cyp11b1 ELISA KIT ,Human CPN1 ELISA KIT ,Human CYP11B ELISA KIT ,Human FHI ELISA KIT ,Human P450C11 ELISA KIT ,Human CYPXIB1 ELISA KIT ,Human cytochrome P-450c11 ELISA KIT ,Human cytochrome P450 11B1 ELISA KIT ,Human  mitochondrial ELISA KIT ,Human cytochrome P450 ELISA KIT ,Human  subfamily XIB steroid 11-beta-hydroxylase ELISA KIT ,Human  polypeptide 1 ELISA KIT ,Human cytochrome P450C11 ELISA KIT ,Human cytochrome p450 XIB1 ELISA KIT ,Human steroid 11-beta-hydroxylase ELISA KIT ,Human steroid 11-beta-monooxygenase ELISA KIT ,Human cytochrome P450 ELISA KIT ,Human  family 11 subfamily B polypeptide 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Cytochrome P450 11B1, mitochondrial, CYP11B1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP11B1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CYP11B1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CYP11B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CYP11B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.