Target Protein Name:Acrosome formation-associated factor(AFAF
Alternative Name:AFAF,Acrosome formation-associated factor(AFAF Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Acrosome formation- associated factor, AFAF ELISA KIT allows for the in vitro quantitative determination of Acrosome formation-associated factor(AFAF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Acrosome formation- associated factor, AFAF ELISA KIT has been pre-coated with an Acrosome formation-associated factor(AFAF antibody specific to Acrosome formation-associated factor(AFAF .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Acrosome formation-associated factor(AFAF and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Acrosome formation-associated factor(AFAF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Acrosome formation-associated factor(AFAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!