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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Acylamino-acid-releasing enzyme,APEH
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Human Acylamino- acid- releasing enzyme, APEH ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Acylamino-acid-releasing enzyme(AARE),Oxidized protein hydrolase(OPH),Acyl-peptide hydrolase(APH),Acylaminoacyl-peptidase,
APEH,AARE; ACPH; APH; D3F15S2; D3S48E; DNF15S2; OPH; N-acylaminoacyl-peptide hydrolase; acyl-peptide hydrolase;
acylamino-acid-releasing enzyme; acylaminoacyl-peptidase; oxidized protein hydrolase; acylaminoacyl-peptide hydrolase
Search name
Human APEH ELISA KIT,Human Acylamino acid releasing enzyme ELISA Kit, Human AARE ELISA Kit, Human Acylamino-acid-releasing enzym ELISA Kit,
Human Oxidized protein hydrolase ELISA Kit, Human OPH ELISA Kit, Human Acyl-peptide hydrolase ELISA Kit, Human APH
ELISA Kit, Human Acylaminoacyl-peptidase ELISA Kit, Human APEH ELISA Kit, Human ACPH ELISA Kit, Human D3F15S2 ELISA
Kit, Human D3S48E ELISA Kit, Human DNF15S2 ELISA Kit, Human N-acylaminoacyl-peptide hydrolase ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human acylamino-acid-releasing enzyme,AARE concentrations
in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AARE. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AARE and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain AARE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AARE in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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