- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- ELI-49359h
- 2025年08月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Apolipoprotein O,APOO
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Human Apolipoprotein O, APOO ELISA KIT
Product Name:Human Apolipoprotein O, APOO ELISA KIT
Packing:96T
Catalog No.:ELI-49359h
Gene Name:APOO
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Apolipoprotein O(APOO
Alternative Name:APOO,Apolipoprotein O(APOO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Apolipoprotein O, APOO ELISA KIT allows for the in vitro quantitative determination of Apolipoprotein O(APOO concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Apolipoprotein O, APOO ELISA KIT has been pre-coated with an Apolipoprotein O(APOO antibody specific to Apolipoprotein O(APOO .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Apolipoprotein O(APOO and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apolipoprotein O(APOO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Apolipoprotein O(APOO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
with patterns that match those of rodent T1α. However, the human data are unclear, because antibodies to rodent T1α do not recognize ATI cell antigens in normal adult human lung. This likely indicates that O-glycosylation of the human protein(s
【分享】Human press METHODS IN MOLECULAR BIOLOGY
, Bret A., 1995 41.Signal Transduction Protocols, 1st. Edited by D. A. Kendall, 1995 42. ELISA Theory and Practice. Edited by Crowther, John R., 1995 43. In Vitro Toxicity Testing Protocols, Edited by O'Hare, Sheila, 1995 44. Agrobacterium
。在ELISA中，DH2一般为无色化合物，经酶作用后成为有色的产物，以便作比色测定。常用的供氢体有邻苯二胺(O-phenylenediamine,OPD)、四甲基联苯胺(3,3'，5,5'-tetramethylbenzidine,TMB)和ABTS[2,2'-azino-di-(3-ethylbenziazobine sulfonate-6)]。 OPD氧化后的产物呈橙红色，用酸终止酶反应后，在492nm处有最高吸收峰，灵敏度高，比色方便，是HRP结合物最常用的底物。OPD本身难溶于水，OPD•2HCL
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