- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-49792h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human 14-3-3 protein eta,YWHAH
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human 14- 3- 3 protein eta, YWHAH ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Protein AS1,YWHAH,14 3 3 protein eta,YWHA1; 14-3-3 eta; 14-3-3 protein eta; tyrosine 3-monooxygenase activation protein, eta polypeptide,tryptophan 5-monooxygenase activation protein eta polypeptide
Search name
Human Protein AS1 ELISA KIT ,Human YWHAH ELISA KIT ,Human 14 3 3 protein eta ELISA KIT ,Human YWHA1 ELISA KIT ,Human 14-3-3 eta ELISA KIT ,Human 14-3-3 protein eta ELISA KIT ,Human tyrosine 3-monooxygenase activation protein eta polypeptide ELISA KIT ,Human tryptophan 5-monooxygenase activation protein eta polypeptide ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human 14- 3- 3 protein eta, YWHAH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YWHAH, During the reaction, YWHAH in the sample or standard competes with a fixed amount of biotin-labeled YWHAH for sites on a pre-coated Monoclonal antibody specific to YWHAH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of YWHAH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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