Human 14-3-3 protein gamma,YWH

Human 14- 3- 3 protein gamma, YWHAG ELISA KIT

Product Name:Human 14- 3- 3 protein gamma, YWHAG ELISA KIT
Packing:96T

Catalog No.:ELI-49766h

Gene Name:YWHAG

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:14-3-3 protein gamma(YWHAG

Alternative Name:YWHAG,14-3-3 protein gamma(YWHAG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human 14- 3- 3 protein gamma, YWHAG ELISA KIT allows for the in vitro quantitative determination of 14-3-3 protein gamma(YWHAG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human 14- 3- 3 protein gamma, YWHAG ELISA KIT has been pre-coated with an 14-3-3 protein gamma(YWHAG antibody specific to 14-3-3 protein gamma(YWHAG .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 14-3-3 protein gamma(YWHAG and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 14-3-3 protein gamma(YWHAG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 14-3-3 protein gamma(YWHAG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!