Human Gamma-adducin,ADD3 ELISA

Human Gamma- adducin, ADD3 ELISA KIT

Product Name:Human Gamma- adducin, ADD3 ELISA KIT
Packing:96T

Catalog No.:ELI-49695h

Gene Name:ADD3

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Gamma-adducin(ADD3

Alternative Name:ADD3,Gamma-adducin(ADD3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Gamma- adducin, ADD3 ELISA KIT allows for the in vitro quantitative determination of Gamma-adducin(ADD3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Gamma- adducin, ADD3 ELISA KIT has been pre-coated with an Gamma-adducin(ADD3 antibody specific to Gamma-adducin(ADD3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gamma-adducin(ADD3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gamma-adducin(ADD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Gamma-adducin(ADD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!