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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Rho GDP-dissociation inhibitor 2,ARHGDIB
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Rho GDP- dissociation inhibitor 2, ARHGDIB ELISA KIT
Product Name:Human Rho GDP- dissociation inhibitor 2, ARHGDIB ELISA KIT
Packing:96T
Catalog No.:ELI-48640h
Gene Name:ARHGDIB
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Rho GDP-dissociation inhibitor 2(ARHGDIB
Alternative Name:ARHGDIB,Rho GDP-dissociation inhibitor 2(ARHGDIB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GDP- dissociation inhibitor 2, ARHGDIB ELISA KIT allows for the in vitro quantitative determination of Rho GDP-dissociation inhibitor 2(ARHGDIB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GDP- dissociation inhibitor 2, ARHGDIB ELISA KIT has been pre-coated with an Rho GDP-dissociation inhibitor 2(ARHGDIB antibody specific to Rho GDP-dissociation inhibitor 2(ARHGDIB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GDP-dissociation inhibitor 2(ARHGDIB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GDP-dissociation inhibitor 2(ARHGDIB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GDP-dissociation inhibitor 2(ARHGDIB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Scale-Up of Single CellInoculated Suspension Cultures of Human Embryonic Stem Cells
by serially upscaling 2-ml static cultures to 50-ml spinner flasks. Use of the Rho kinase (ROCK) inhibitor Y-27632 (Ri) was found to enable cell survival in suspension by promoting hESC reaggregation. This treatment resulted in 44% (vs. 5–10% in untreated
of hESC for the MTT Assay Using the Single‐Cell Method with Rock Inhibitor Alternate Protocol 1: Preparation of Human Embryonic Stem Cells for the MTT Assay Using the Small‐Colony Method Support Protocol 5: Assay
Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
a standard alkaline phosphatase staining kit to reveal expression of the pluripotent stem cell marker APLP. Note that while hCD29 is expressed by all human pluripotent stem cells throughout each colony (E), this antibody fails to stain live cells
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