FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
KHNYN,protein KHNYN; KIAA0323; KH and NYN domain containing
Search name
Human KHNYN ELISA KIT ,Human protein KHNYN ELISA KIT ,Human KIAA0323 ELISA KIT ,Human KH and NYN domain containing ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Protein KHNYN, KHNYN concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein KHNYN, During the reaction, Protein KHNYN in the sample or standard competes with a fixed amount of biotin-labeled Protein KHNYN for sites on a pre-coated Monoclonal antibody specific to Protein KHNYN. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein KHNYN in the samples is then determined by comparing the O.D. of the samples to the standard curve.