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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Ecto-NOX disulfide-thiol exchanger 2,ENOX2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human Ecto-NOX disulfide-thiol exchanger 2, ENOX2 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Human ENOX2 ELISA KIT ,Human Ecto-NOX disulfide-thiol exchanger 2 ELISA KIT ,Human CT5.2 ELISA KIT ,Human CT5.2A ELISA KIT ,Human HD21 ELISA KIT ,Human HOM-MEL-40 ELISA KIT ,Human SSX ELISA KIT ,Human cancer/testis antigen 5.2 ELISA KIT ,Human cancer/testis antigen family 5 member 2a ELISA KIT ,Human sarcoma synovial X-chromosome-related 2 ELISA KIT ,Human synovial sarcoma X breakpoint 2B ELISA KIT ,Human tumor antigen HOM-MEL-40 ELISA KIT ,Human synovial sarcoma X breakpoint 2 ELISA KIT
Intended use
Human ENOX2 ELISA kit allows for the in vitro quantitative determination of Human Ecto-NOX disulfide-thiol exchanger 2, ENOX2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Human ENOX2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ENOX2, During the reaction, ENOX2 in the sample or standard competes with a fixed amount of biotin-labeled ENOX2 for sites on a pre-coated Monoclonal antibody specific to ENOX2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ENOX2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 0.31-20ng/ml
Human ENOX2 ELISA kit Manual download
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文献和实验cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
secondary antibody review -- data from 99 publications
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Analysis of Oxidative Modification of Proteins
. Except for cysteine residues, whose oxidation is often determined by the loss of protein thiol groups, quantification of oxidative damage to tyrosine, and aspartate residues is usually carried out by the measurement of specific oxidation products such as dityrosine
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