- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-46903h
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Dolichol kinase,DOLK
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human Dolichol kinase, DOLK ELISA KIT
Product Name:Human Dolichol kinase, DOLK ELISA KIT
Packing:96T
Catalog No.:ELI-46903h
Gene Name:DOLK
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Dolichol kinase(DOLK
Alternative Name:DOLK,Dolichol kinase(DOLK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Dolichol kinase, DOLK ELISA KIT allows for the in vitro quantitative determination of Dolichol kinase(DOLK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Dolichol kinase, DOLK ELISA KIT has been pre-coated with an Dolichol kinase(DOLK antibody specific to Dolichol kinase(DOLK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Dolichol kinase(DOLK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dolichol kinase(DOLK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Dolichol kinase(DOLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S. 2002b. The protein kinase complement of the human genome. Science 298:1912‐1934.
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
DNA (Stratagene) Plasmid Midi kit (QIAGEN) Proteinase K (Roche Applied Science) Recombinant Hop2-Mnd1 complex, purified (5 µM) or recombinant human Rad54, purified (5 µM) (see Step 5) Purify Hop2-Mnd1 complex
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