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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Cytochrome c oxidase assembly factor 5,COA5
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Cytochrome c oxidase assembly factor 5, COA5 ELISA KIT
Product Name:Human Cytochrome c oxidase assembly factor 5, COA5 ELISA KIT
Packing:96T
Catalog No.:ELI-46853h
Gene Name:COA5
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Cytochrome c oxidase assembly factor 5(COA5
Alternative Name:COA5,Cytochrome c oxidase assembly factor 5(COA5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cytochrome c oxidase assembly factor 5, COA5 ELISA KIT allows for the in vitro quantitative determination of Cytochrome c oxidase assembly factor 5(COA5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cytochrome c oxidase assembly factor 5, COA5 ELISA KIT has been pre-coated with an Cytochrome c oxidase assembly factor 5(COA5 antibody specific to Cytochrome c oxidase assembly factor 5(COA5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cytochrome c oxidase assembly factor 5(COA5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome c oxidase assembly factor 5(COA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cytochrome c oxidase assembly factor 5(COA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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Typical transformation results
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【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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