- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-46382h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human M-phase inducer phosphatase 3,CDC25C
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human M- phase inducer phosphatase 3, CDC25C ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC25C,dual specificity phosphatase CDC25C; CDC25; PPP1R60; M-phase inducer phosphatase 3; mitosis inducer CDC25; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60; cell division cycle 25C
Search name
Human CDC25C ELISA KIT ,Human dual specificity phosphatase CDC25C ELISA KIT ,Human CDC25 ELISA KIT ,Human PPP1R60 ELISA KIT ,Human M-phase inducer phosphatase 3 ELISA KIT ,Human mitosis inducer CDC25 ELISA KIT ,Human phosphotyrosine phosphatase ELISA KIT ,Human protein phosphatase 1 ELISA KIT ,Human regulatory subunit 60 ELISA KIT ,Human cell division cycle 25C ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human M- phase inducer phosphatase 3, CDC25C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to M-phase inducer phosphatase 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-phase inducer phosphatase 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain M-phase inducer phosphatase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of M-phase inducer phosphatase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
in the developing striatum. Neuron 9 :15. Henthorn, P., P. Zervos, M. Raducha, H. Harris, and T. Kadesch. 1988. Expression of a human placental alkaline phosphatase gene in transfected cells: Use as a reporter for studies of gene expression. Proc. Natl
Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling
in an inactive form (pre-MPF) during the G2 arrest owing to the phosphorylation of Cdc2 by the Myt1 protein kinase. The dephosphorylation necessary to activate pre-MPF and induce progression of the oocyte into meiosis is catalyzed by the phosphatase Cdc25C.
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