- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-46116h
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Nucleolar protein 10,NOL10
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human Nucleolar protein 10, NOL10 ELISA KIT
Product Name:Human Nucleolar protein 10, NOL10 ELISA KIT
Packing:96T
Catalog No.:ELI-46116h
Gene Name:NOL10
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Nucleolar protein 10(NOL10
Alternative Name:NOL10,Nucleolar protein 10(NOL10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Nucleolar protein 10, NOL10 ELISA KIT allows for the in vitro quantitative determination of Nucleolar protein 10(NOL10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Nucleolar protein 10, NOL10 ELISA KIT has been pre-coated with an Nucleolar protein 10(NOL10 antibody specific to Nucleolar protein 10(NOL10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Nucleolar protein 10(NOL10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nucleolar protein 10(NOL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Nucleolar protein 10(NOL10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验which provide close-ups of certain steps of the protocol. Special Feature: Complete nucleolar isolation protocol in one Quicktime movie Click here to view at high resolution (25.8 Mb) Warning
in the same volume of Buffer 1 as the initial volume transferred from the vial in step 2. 2. Sample Preparation Preparation of MNC from Buffy Coat to Obtain Low Platelet Numbers 1) Dilute 10 – 18 ml buffy coat with Buffer 2 to a total
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
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