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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Polymerase delta-interacting protein 3,POLDIP3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Polymerase delta- interacting protein 3, POLDIP3 ELISA KIT
Product Name:Human Polymerase delta- interacting protein 3, POLDIP3 ELISA KIT
Packing:96T
Catalog No.:ELI-45841h
Gene Name:POLDIP3
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Polymerase delta-interacting protein 3(POLDIP3
Alternative Name:POLDIP3,Polymerase delta-interacting protein 3(POLDIP3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Polymerase delta- interacting protein 3, POLDIP3 ELISA KIT allows for the in vitro quantitative determination of Polymerase delta-interacting protein 3(POLDIP3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Polymerase delta- interacting protein 3, POLDIP3 ELISA KIT has been pre-coated with an Polymerase delta-interacting protein 3(POLDIP3 antibody specific to Polymerase delta-interacting protein 3(POLDIP3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Polymerase delta-interacting protein 3(POLDIP3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Polymerase delta-interacting protein 3(POLDIP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Polymerase delta-interacting protein 3(POLDIP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to establish, by using separate assays, that the interacting regions captured in the 6C assay are truly occupied by the protein of interest in cells. To this end, perform ChIP assays (Tiwari et al. 2008) using antibodies specific for the protein
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
DNA purification kit (e.g., QIAquick Spin Miniprep Kit, Qiagen; optional) 2.5 U/µl proofreading PCR polymerase (e.g., Deep Vent; New England Biolabs) and 10× buffer
【资源】[共享]Adhesion Protein Protocols——(很有用的)
Contents: Table of Contents Adhesion Protein Protocols Preface Contents Contributors 1— Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules Selective Protocols of Immunizati... 1— Introduction 2— Materials 3— Methods 4—
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