Human Protein disulfide- isomerase A4, PDIA4 ELISA KIT
Product Name:Human Protein disulfide- isomerase A4, PDIA4 ELISA KIT Packing:96T
Catalog No.:ELI-45840h
Gene Name:PDIA4
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Protein disulfide-isomerase A4(PDIA4
Alternative Name:PDIA4,Protein disulfide-isomerase A4(PDIA4 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Protein disulfide- isomerase A4, PDIA4 ELISA KIT allows for the in vitro quantitative determination of Protein disulfide-isomerase A4(PDIA4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Protein disulfide- isomerase A4, PDIA4 ELISA KIT has been pre-coated with an Protein disulfide-isomerase A4(PDIA4 antibody specific to Protein disulfide-isomerase A4(PDIA4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein disulfide-isomerase A4(PDIA4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein disulfide-isomerase A4(PDIA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein disulfide-isomerase A4(PDIA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!