- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-44867h
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Nuclear pore complex protein Nup153,NUP153
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human Nuclear pore complex protein Nup153,NUP153 ELISA KIT
Product Name:Human Nuclear pore complex protein Nup153,NUP153 ELISA KIT
Packing:96T
Catalog No.:Human NUP153 ELISA KIT
Gene Name:NUP153
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Human NUP153
Alternative Name:Human NUP153
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NUP153 ELISA KIT allows for the in vitro quantitative determination of Human NUP153 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NUP153 ELISA KIT has been pre-coated with an Human NUP153 antibody specific to Human NUP153 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human NUP153 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NUP153, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human NUP153 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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at 4°C. Transfer supernatant (nuclear fraction) to a clean microcentrifuge tube. 11. Aliquot and store at –80°C. The nuclear extracts are ready for assay. 12. Quantitate protein concentration using the Quant-iT™ Protein Quantitation Kit (Cat. no. Q
Reconstitution of Nuclear Import in Permeabilized Cells
factors. The latter include receptors (karyopherins) that recognize the cargoes and carry them across the nuclear pore complex (NPC), and the small GTPase Ran, which modulates karyopherin–cargo binding. Nuclear import can be studied in vitro using
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