- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-44761h
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ubiquitin-conjugating enzyme E2 N,UBE2N
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human Ubiquitin-conjugating enzyme E2 N,UBE2N ELISA KIT
Product Name:Human Ubiquitin-conjugating enzyme E2 N,UBE2N ELISA KIT
Packing:96T
Catalog No.:Human UBE2N ELISA KIT
Gene Name:UBE2N
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Human UBE2N
Alternative Name:Human UBE2N
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human UBE2N ELISA KIT allows for the in vitro quantitative determination of Human UBE2N concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human UBE2N ELISA KIT has been pre-coated with an Human UBE2N antibody specific to Human UBE2N .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human UBE2N and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human UBE2N, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human UBE2N in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
in Insect Cells Using a Baculovirus System Alternate Protocol 11: Preparation of Rabbit E1 Support Protocol 4: Generation of Empty Bacterial Lysates Generation of Ubiquitin‐Conjugating Enzymes (E2s) Basic Protocol 22: Expression of E2s in E. coli
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
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