- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-44222h
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Nuclear pore complex protein Nup214,NUP214
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human Nuclear pore complex protein Nup214,NUP214 ELISA KIT
Product Name:Human Nuclear pore complex protein Nup214,NUP214 ELISA KIT
Packing:96T
Catalog No.:Human NUP214 ELISA KIT
Gene Name:NUP214
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Human NUP214
Alternative Name:Human NUP214
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NUP214 ELISA KIT allows for the in vitro quantitative determination of Human NUP214 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NUP214 ELISA KIT has been pre-coated with an Human NUP214 antibody specific to Human NUP214 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human NUP214 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NUP214, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human NUP214 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验施一公团队突破性进展再登 Science！以超高分辨率解析核孔复合物的结构细节
分辨率为 5.5Å，包含 Nup358 区域 7.1 Å，Nup214 区域 7.9 Å，局部分辨率达 4.5 Å。在如此高分辨率下，更加清晰准确地将基本的蛋白二级结构和大部分的 CR 组分修入模型当中。 图片来源：Cell Research 2022 年 2 月 17 日，施一公团队在 Cell Research 在线发表题为 Cryo-EM structure of the nuclear ring from Xenopus laevis nuclear pore complex 的研究论文，该研究展示
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
at 4°C. Transfer supernatant (nuclear fraction) to a clean microcentrifuge tube. 11. Aliquot and store at –80°C. The nuclear extracts are ready for assay. 12. Quantitate protein concentration using the Quant-iT™ Protein Quantitation Kit (Cat. no. Q
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